The composition of egg yolk media is important for the identification of egg yolk positive strains of coagulase positive staphylococci. Media with glucose allowed the detection of more egg yolk positive strains than those without it. However, the presence of 50% CO 2 in the incubation atmosphere allowed a greater number of egg yolk positive strains to be detected regardless of whether glucose was present or not. This was due to the suppression of lipase activity by CO 2 thus allowing total lipid separation indicative of the egg yolk phenomenon. Staphylococcus aureus strains from human nares were egg yolk positive and strongly lipolytic while strains of animal origin and epidermal necrolysis were egg yolk negative and weakly lipolytic.
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Owens et al. (1975) studied this question.
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