Essential oils (EO) from leaves of Ocimum gratissimum L., Ocimum micranthum Willd.and Ocimum selloi Benth.obtained by steam distillation (SD), microwave oven distillation (MO) and supercritical extraction with CO 2 (SC) showed different composition by GC/MS analysis.The main components in the three essential oils were the same but in different relative amounts.The oil from O. gratissimum obtained by SD presented eugenol (54.0%) and 1,8-cineole (21.6%) as main components, by MO eugenol (34.6%) and 1,8-cineole (22.6%), and by SC eugenol (73.1%) and 1,8-cineole (5.6%).The essential oil from O. micranthum obtained by SD showed mainly eugenol (64.8 %), (E)-β-caryophyllene (14.3%) and bicyclogermacrene (8.1%); by MO eugenol (30.6 %), trans-β-caryophyllene (19.8 %) and elemicin (19.0), and the oil obtained by SC presented eugenol (56.7%), elemicin (16.0%) and trans-β-caryophyllene (4.0%).Essential oil of O. selloi obtained by SD presented anethole (64.6%) and linalool (20.6%); by MO anethole (67.4%) and linalool (20.9%) and by SC anethole (31.9%) and linalool (23.2%) as main constituents.The type and quantitative variations of the chemical components of the analyzed oils in relation to the extraction methods were greater for O. micranthum.
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Silva et al. (2004) studied this question.