In vitro cultivation of parthenogenesis‐inducing Wolbachia in an Aedes albopictus cell line

Parthenogenesis‐inducing (PI) Wolbachia, infecting the parasitoid wasp Trichogramma kaykai Pinto and Stouthamer (Hymenoptera: Trichogrammatidae), were successfully maintained and cultivated in a mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae), cell line, NIAS‐AeAl‐2. A parthenogenesis‐inducing (PI) Wolbachia strains with wasp ovaries were introduced into flasks with cultures of the cell line. The PI Wolbachia proliferated in NIAS‐AeAl‐2 as confirmed using a quantitative polymerase chain reaction method. Our final goal is to induce parthenogenesis in other hymenopteran species of commercial interest through the artificial transfer of PI Wolbachia. As a step towards this goal, we microinjected pupae of Trichogramma with PI Wolbachia culture in NIAS‐AeAl‐2 cells and PI Wolbachia was detected from recipient by PCR with Wolbachia specific primers (wsp gene).