Methods of isolating the microflora of moulding, high ‐moisture barley in partially sealed silos

SUMMARY Fungi were isolated on agar from barley stored at 18–44% moisture content in partially sealed silos by plating out whole, untreated grain as removed from the silos; surface‐washed whole grain; surface‐washed and then sodium hypochlorite‐sterilized whole grain; hypochlorite surface‐sterilized, dehusked grains cut vertically and plated with cut surfaces to the agar; husks washed several times after removal and plated as small groups. 5 mg aureomycin/100 ml agar was used to suppress bacterial growth. These methods are compared with others in common use. The isolated species have been tabulated to illustrate the various combinations occurring in grains and in aggregates of husks.