uMMARY Conditions for the neutralization test of \, aricella-zoster (V-Z) virus were investigated in detail for the purpose of making this test sufficiently simple and efficient for' routine use.It \\, as found that the number of PIaques produced under liquid medium 7 to 8 days after infection \\, as coinparable with the number produced under solid medium, and that no secondary PIaques under liquid medium appeared during a 14-day observation period.Thus PIaque counting under liquid medium at 7 to 8 day after infection \\, as a convenient method for assay of viral infectivity and for the ncutralization (NT) test.PSGC medium (PBS(-) containing 51h sucrose, 0.11, Na-glutamate and 1011 fetal calf serum) was found suitable for keeping infectivity of V-Z \, irus as stable as possible.\\'ith this medium as \, irus and serum diluent, the most efficient conditions for the NT test are incubation of serum-virus mixture for 30 to 60 min at 37 C with occasional shaking.In a serological follow-up of cases of varicella and zoster, all4 cases of zoster examined were found to have high NT antibody titers I to 2 \\, eeks before the onset of the disease, suggesting that decrease in the NT antibody titer may not be the cause of development of zoster symptoms.
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Asano et al. (1978) studied this question.