Increased p53 Protein Does Not Correlate to p53 Gene Mutations in Microdissected Human Testicular Germ Cell Tumors

No AccessJournal of UrologyInvestigative Urology1 Aug 1995Increased p53 Protein Does Not Correlate to p53 Gene Mutations in Microdissected Human Testicular Germ Cell Tumors Noah S. Schenkman, Isabell A. Sesterhenn, Lucille Washington, Yue Ao Tong, Christopher M. Weghorst, Gregory S. Buzard, Shiv Srivastava, and Judd W.* Moul Noah S. SchenkmanNoah S. Schenkman More articles by this author , Isabell A. SesterhennIsabell A. Sesterhenn More articles by this author , Lucille WashingtonLucille Washington More articles by this author , Yue Ao TongYue Ao Tong More articles by this author , Christopher M. WeghorstChristopher M. Weghorst More articles by this author , Gregory S. BuzardGregory S. Buzard More articles by this author , Shiv SrivastavaShiv Srivastava More articles by this author , and Judd W.* MoulJudd W.* Moul More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)67122-2AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To determine if primary testicular germ cell tumors that overexpress p53 tumor suppressor gene protein have p53 gene mutations. Materials and Methods: We examined 30 primary testicular tissues from 26 patients representing two groups. Group one consisted of eleven cases (6 nonseminomatous germ cell tumors and 5 seminomas) in which tissue samples for DNA analysis were microdissected from paraffin block regions with elevated immunohistochemical staining for p53 protein. Group two consisted of 19 testis tumor tissues which had been fresh frozen and were chosen to correspond to archival tissue specimens exhibiting elevated levels of p53 protein. The DNA was extracted from these tissues and subjected to exon specific amplification by polymerase chain reaction (PCR) and cold single-strand conformation polymorphism (Cold SSCP) analysis. Results: In these cases with elevated p53 protein, no p53 gene exon 5-8 mutations were detected except 1 seminoma with a codon 140 silent mutation (no protein alteration). Conclusions: Testicular tumors appear to exhibit elevated levels of wild-type p53 protein, the significance of which is yet to be elucidated. References 1 : Germ cell tumors of testis: prognostic factors and results. Eur. Urol.1990; 18: 242. Google Scholar 2 : Neoplasms of the testis. In: Campbell's Urology. 6th edition. Edited by . Philadelphia: W. B. Sanders Co.1992: 1222. Google Scholar 3 : Treatment of testicular cancer: a new and improved model. J. 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Google Scholar Urology Service, Department of Surgery, Walter Reed Army Medical Center, Washington, D.C., the Center for Prostate Disease Research, Uniformed Services University of the Health Sciences, Bethesda, Maryland, the Armed Forces Institute of Pathology, Washington, D.C., the Laboratory of Comparative Carcinogenesis, N.C.I., F.C.R.D.C., Frederick, Maryland and B.C.D.P., PRI/Dyn Corporation, Frederick, Maryland.© 1995 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byMoul J (2018) Molecular Genetics of Testicular CancerJournal of Urology, VOL. 157, NO. 5, (1552-1553), Online publication date: 1-May-1997.Sandberg A, Meloni A and Suijkerbuijk R (2018) Reviews of Chromosome Studies in Urological Tumors. III. Cytogenetics and Genes in Testicular TumorsJournal of Urology, VOL. 155, NO. 5, (1531-1556), Online publication date: 1-May-1996. Volume 154 Issue 2 August 1995 Page: 617-621 Advertisement Copyright & Permissions© 1995 by American Urological Association, Inc.Metrics Author Information Noah S. Schenkman More articles by this author Isabell A. Sesterhenn More articles by this author Lucille Washington More articles by this author Yue Ao Tong More articles by this author Christopher M. Weghorst More articles by this author Gregory S. Buzard More articles by this author Shiv Srivastava More articles by this author Judd W.* Moul More articles by this author Expand All Advertisement PDF downloadLoading ...