What question did this study set out to answer?

To solubilize, partially purify, and characterize membrane-associated cross-protection factors from in vivo-grown Pasteurella multocida.

October 1, 1994Avian Diseases

Partial Purification of Cross-Protection Factor(s) from Pasteurella multocida

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Authors

RRR. B. RimlerAgricultural Research Service

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Implication

Experimental study demonstrates cross-protection against Pasteurella multocida via specific membrane proteins and carbohydrates in turkeys, indicating multifaceted antigen requirements for immunity.

Key Points

To solubilize, partially purify, and characterize membrane-associated cross-protection factors from in vivo-grown Pasteurella multocida.
Extracted membrane-associated factors from in vivo-grown bacteria with detergent and separated them sequentially using ion exchange, gel filtration, and hydroxyapatite chromatography.
Assessed protection by challenging actively vaccinated turkeys and poults passively immunized with serum from vaccinated turkeys.
Analyzed active chromatographic fractions using SDS-PAGE, immunoblots, and periodate oxidation treatment.
Protective chromatography fractions contained prominent protein bands at 39 kDa and within the 59–65 kDa molecular mass range.
Treatment of protective fractions with periodate significantly diminished cross-protection, indicating that carbohydrate determinants contribute to protective immunity alongside proteins.

Cite This Study

R. B. Rimler (1994) studied this question.

synapsesocial.com/papers/6a9fc99040bc1a697f1de569 https://doi.org/10.2307/1592114

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Implication

Experimental study demonstrates cross-protection against Pasteurella multocida via specific membrane proteins and carbohydrates in turkeys, indicating multifaceted antigen requirements for immunity.

Partial Purification of Cross-Protection Factor(s) from Pasteurella multocida

Discussion

Member takes

Implication

Key Points

Cite This Study

Partial Purification of Cross-Protection Factor(s) from Pasteurella multocida

Discussion

Member takes

Implication

Key Points

Cite This Study

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