Answers are grounded in this paper and cite their sources.
In this study, we developed a fluorescence-based assay for quantifying the aromatic amino acid L-tyrosine in small sample volumes employing 96-well microtiter plates. The method was based on the specific derivatisation of L-tyrosine with 1-nitroso-2-naphthol and the formation of a stable complex for the fluorescent determination of L-tyrosine concentrations. The original procedure for L-tyrosine measurements in blood or tissue samples (Waalkes and Udenfriend in J Lab Clin Med 50:733-736, 1957) was modified to a simple assay suitable for high-throughput screening of L-tyrosine producing microorganisms such as engineered Escherichia coli.
No takes yet. Share an insight, caveat, or question.
Lütke‐Eversloh et al. (2007) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: