December 10, 1999Human Gene TherapyOpen Access

Intramyocardial Gene Therapy with Naked DNA Encoding Vascular Endothelial Growth Factor Improves Collateral Flow to Ischemic Myocardium

Q: What is the clinical evidence from this study?

Population: chronic myocardial ischemia (n=36). Intervention: phVEGF165 (naked DNA encoding VEGF) vs. pCMVbeta (plasmid DNA encoding beta-galactosidase). Primary outcome: Maximal vasodilatation flow to the ischemic region (p=< 0.05).

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Key result

Direct intramyocardial injection of phVEGF165 significantly increased maximal vasodilatation flow to the ischemic region in a porcine model compared to controls (2.16 vs 1.31; p<0.05).

Why the study?

Does direct intramyocardial injection of naked DNA encoding VEGF improve myocardial perfusion in a porcine model of chronic myocardial ischemia?

Population

36 pigs

Comparison

Direct intramyocardial injection of 500 microg… vs Direct intramyocardial injection of 200 microg…

Design

Preclinical

Authors

RTRené A. TioCardiac Imaging TTTengis TkebuchavaTufts University TSThomas H. ScheuermannThe University of Texas Southwestern Medical Center

Discussion

Member takes

Implication

Positive porcine data remain hypothesis-generating; leaves open human translation of intramyocardial VEGF gene therapy.

Structured PICO

Does direct intramyocardial injection of naked DNA encoding VEGF improve myocardial perfusion in a porcine model of chronic myocardial ischemia?

Population

36 pigs (23 nonischemic, 13 ischemic) evaluated for myocardial perfusion after intramyocardial injection of naked DNA encoding VEGF.

Intervention

Direct intramyocardial injection of 500 microg of naked DNA encoding for VEGF (phVEGF165) into four separate sites

Comparator

Direct intramyocardial injection of 200 microg of plasmid DNA encoding beta-galactosidase (pCMVbeta)

Outcome

Myocardial perfusion (resting myocardial blood flow and maximal vasodilatation with adenosine) and collateral fillingsurrogate

Main Result

Absolute Event Rate: 2.16% vs 1.31%

p-value: p=< 0.05

Direct intramyocardial transfection of naked DNA encoding VEGF is safe and enhances collateral formation and myocardial perfusion in a porcine model of chronic myocardial ischemia.

Cite This Study

Tio et al. (1999) studied chronic myocardial ischemia (n=36). phVEGF165 (naked DNA encoding VEGF) vs. pCMVbeta (plasmid DNA encoding beta-galactosidase) was evaluated on Maximal vasodilatation flow to the ischemic region (p=< 0.05). Direct intramyocardial injection of phVEGF165 significantly increased maximal vasodilatation flow to the ischemic region in a porcine model compared to controls (2.16 vs 1.31; p<0.05).

synapsesocial.com/papers/6a9fd6021f0ba8eae6bab75e https://doi.org/10.1089/10430349950016366

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Intramyocardial Gene Therapy with Naked DNA Encoding Vascular Endothelial Growth Factor Improves Collateral Flow to Ischemic Myocardium

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Intramyocardial Gene Therapy with Naked DNA Encoding Vascular Endothelial Growth Factor Improves Collateral Flow to Ischemic Myocardium

Discussion

Member takes

Implication

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider