Two cell lines of C3Hf mouse embryo cells were tested for the effect of various sera on neoplastic conversion and chromosomal alteration in number and morphology. The sera, added to chemically defined medium NCTC 135, included horse, fetal calf, calf, bovine, and a combination of horse and fetuin. In both lines, cells in horse and calf serum underwent neoplastic conversion earlier than in fetal calf serum. In one line cells in bovine serum also underwent the early conversion. The results suggested that fetuin might delay the neoplastic conversion, but further data are needed to establish this point. Calf and bovine sera, like fetal calf serum, reduced the rate of heteroploidization and appearance of abnormal chromosomes as compared with horse serum. Thus, the calf and bovine sera apparently contain some factor(s) common to fetal calf serum or lack the factor(s) in horse serum that influences chromosomal stability. However, the calf serum effects neoplastic conversion to the same extent as horse serum. From the distribution and morphology of the abnormal chromosomes, we conclude that the chromosome breaks during in vitro growth in all sera tested are at or near the centromere.
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Parshad et al. (1968) studied this question.