In vitro study reveals temperature and substrate significantly alter endodontic sealer flow and wetting (p < 0.001), indicating body heat dictates clinical sealing behavior.
Background: This study aimed to investigate flow, contact angles and capillary penetration of commercially available endodontic sealers by substrate (human root dentin discs [HRD] and gutta-percha [GP]) and by temperature [room (20 °C), or body (37 °C)]. Methodology: Nine commercially available endodontic sealers were tested using the two-plate method, contact angle calculation (measured on HRD and GP surface—for both temperatures), free-flow, and penetration co-efficiency using a capillary permeability test (in 37 °C). Discs, set-ups and sealers were prepared according to the protocol; specimens were photographed; results were collected; and all recordings were statistically analyzed. Results: The chemical composition of each sealer significantly affected rheological properties even in the same chemical group (p < 0.001). Also, recordings from every test made were also significantly influenced by temperature (p < 0.001). More specifically, the behaviour of sealers under higher temperature proved to be sealer-specific. Conclusions: Within the limitation of this study, it was verified that Endodontic sealers’ rheological properties were affected by the substrate’s composition and temperature. Additionally, body temperature significantly influenced thier performance.
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Sotiropoulos et al. (2026) studied this question.
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