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September 8, 2026Methods and ProtocolsOpen Access

Unveiling Distinct Developmental and Cellular Impacts of Cisplatin on Zebrafish (Danio rerio) Embryos: Application of a Combined Developmental Toxicity with Rapid DNA-Content Flow Cytometry Protocol

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Authors

MLMaximos I. LeonardosVKVasileia KaravidaATAnna Tsoukaneli

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Overview

Experimental study reveals dose-dependent growth reduction and G2/M arrest in cisplatin-treated zebrafish embryos, highlighting rapid flow cytometry for whole-embryo toxicity screening.

Key Points

  • To evaluate the developmental, behavioral, and cellular toxicity of cisplatin in zebrafish embryos using an adapted single-embryo rapid DNA-content flow cytometry workflow.
  • Exposed dechorionated zebrafish (Danio rerio) embryos to 10–400 μM cisplatin for 96 hours.
  • Evaluated developmental endpoints, including body length, eye surface area, heart rate, locomotor activity, thigmotaxis, and touch-evoked and vibrational startle responses.
  • Quantified individual whole-embryo cell-cycle distribution without sample pooling using rapid mechanical dissociation, propidium iodide/RNase staining, and flow cytometry.
  • Cisplatin induced time- and concentration-dependent reductions in embryonic growth and high-dose behavioral alterations, while heart rate remained comparatively unaffected.
  • Flow cytometric analysis demonstrated pronounced cell-cycle redistribution at 400 μM cisplatin, characterized by a significant decrease in the G0/G1 phase and an increase in the G2/M phase.

Cite This Study

Leonardos et al. (2026) studied this question.

synapsesocial.com/papers/6a9fd82f58e84d0ff5b47798https://doi.org/10.3390/mps9050132
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