Procedure for the isolation of lipases produced by Pseudomonas species and Achromobacter lipolyticum

The extracellular lipase systems from three Pseudomonas species and a strain of Achromobacter lipolyticum were concentrated and partially purified using a D.D.S. plate-and-frame ultrafiltration unit. Recoveries ranging from 75–97% were reported for the pseudomonad enzyme systems with increases in specific activity of 18–40 times. The low yields obtained for Achromobacter lipolyticum preparations were concluded to be due to the loss of a loosely bound ion or low molecular weight compound necessary for optimum activity.