Chemical synthesis of γ-echistatin analogues: elucidation of status of C-terminal (45–49)

Three analogues of gamma-echistatin, des(45-49)-gamma-echistatin, des(46-49)-gamma-echistatin and des(47-49)-gamma-echistatin, were synthesized by solid-phase methodology and their biological activities were measured and compared. The results reveal that without the C-terminal (45-49) of gamma-echistatin, the folding of the protein to the final active structure is not interfered with and Lys-45 influences the inhibition of platelet aggregation.