SYNOPSIS. Chloramphenicol cured Crithidia deanei of its endosymbiote. The derived aposymbiotic strain had additional growth requirements: purin (as adenine), heme, arginine, histidine, isoleucine, leucine, phenylalanine, threonine, tryptophan, valine, pyridoxine, riboflavin, and pantothenate and liver infusion (replaceable by high nicotinamide).
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MUNDIM et al. (1977) studied this question.
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