Molecular Genetic Analysis of a B^3 Allele in a Rare Subtype A_1B_3 Blood-type Individual

Molecular genetic analysis of a rare subtype, A_1B_3, is reported. The targets of nucleotide alterations were within exons 6 and 7. After sequencing analysis, two typical SNPs of 547G>A and 467C>T were observed. Adenine nucleotide at nt 547 was identified as B^ (ref. Cho et al., 2004) on the B allele in an A_1B_3 by ASP-PCR-RFLP without a cloning procedure. This variant could be rapidly identified by the ASP-PCR with a variant-specified primer. The patterns of PCR-SSCP near nt 547 and 467 were altered. Finally this A_1B_3 individual was identified as A^<1v>B^3.