A photobleaching model: D-P (dye-photon interaction) and D-O (Dye-oxygen oxidative reaction) photobleaching theory, is proposed. The quantitative power dependences of photobleaching rates with both one- and two-photon excitations (1 PE and TPE) are obtained. This photobleaching model can be used to elucidate our and other experimental results commendably. Experimental studies of the photobleaching rates for rhodamine B with TPE under unsaturation conditions reveals that the power dependences of photobleaching rates increase with the increasing dye concentration, and that the photobleaching rate of a single molecule increases in the second power of the excitation intensity, which is different from the high-order (>3) nonlinear dependence of ensemble molecules.
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Chen et al. (2003) studied this question.
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