Serum Trace Minerals Profile in Anestrus Buffaloes Treated With Different Estrus Synchronization Protocols

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The present study was conducted to analyse the serum trace minerals (copper, cobalt, iron, zinc, manganese and selenium) concentration in postpartum anestrus buffaloes. A total of 24 postpartum anestrus buffaloes were randomly selected after taking reproductive history and gynaeco-clinical examination 10 days apart. These 24 animals were divided into four groups; Group A (Ovsynch), Group B (Heatsynch) and Group C (CIDR plus Ovsynch) and Control group (no treatment) each containing six animals. Blood sample was collected on day 0, 7, 9 and 10 of treatment and serum was analysed for various trace minerals. The mean serum copper level (µg/ml) increased significantly (p<0.01) from day 0 to day 7, 9 and 10 in group A, B and C. The mean serum cobalt concentration increased significantly (p<0.05) from day 0 to day 7, 9 and 10 in group A, B and C. Serum iron concentration increased significantly (p<0.05) from day 0 to day 9 and 10 except on day 7 in group A, B and C and on day 9 in group A. The mean zinc level (µg/ml) increased significantly (p<0.05) in group B and C on day 9 and 10 except day 7. The serum manganese concentration on day 0 increased significantly (p<0.01) to day 9 and 10, the value on day 0 was also found significant from day 7 in group C. The mean serum selenium, the value on day 0 increased significantly (p<0.01) to day 7, 9 and 10 in group A, B and C except the value on day 7 in group B. Thus, it could be concluded that deficiency of copper, iron, zinc and selenium either alone or in combination could be responsible for anestrus condition. By improving the trace minerals status, fertility can be improved in buffaloes.