Enzymatic Determination of Serum Adenosine-5-Triphosphate by Amperometric Measurement of the Rate of Oxygen Depletion

ATP is determined by measuring the competition for glucose between a hexokinase-catalyzed dephospnorylation of ATP and glucose oxidase catalyzed oxidation of glucose. The ATP competition for glucose decreases the rate of oxygen consumption via the oxidation reaction, which is measured amperometrically with a Clark oxygen electrode. The decrease in the oxygen depletion is proportional to the concentration of ATP in the sample. A 10 to 50 μl sample is required and analyses are completed in 3 minutes or less.