Answers are grounded in this paper and cite their sources.
A laboratory application with a strong component in analytical chemistry was designed for undergraduate students, in order to introduce a current problem in the environmental science field, the water contamination by antibiotics. Therefore, a simple and rapid method based on direct injection and high performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed and optimized for the determination of amoxicillin in river water. Students learned the main optimization steps for the improvement of the LC–MS/MS methodology and the correct procedure to validate an analytical method (draw a calibration curve, determine detection and quantification limits, precision, and accuracy). This laboratory experiment was successfully applied by students and enables the analysis of a large number of samples in a short period of time because of the short run time (3 min).
No takes yet. Share an insight, caveat, or question.
Homem et al. (2014) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: