A technique for the application of dieca (diethyldithiocarbamate) to plant tissues is described. It is shown to differentiate almost quantitatively between extracted ascorbic oxidase and polyphenolase on the one hand and cytochrome oxidase on the other at a concentration of M/5,000. It produces strong, but not total, inhibitions when applied to barley root-tips.
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JAMES et al. (1952) studied this question.