Fast and furious: A rapid and inexpensive assay for determining both the activity and enantioselectivity of ketoreductases (KREDs) has been developed (see scheme; HRP=horseradish peroxidase, ABTS=2,2′-azino-di(3-ethyl benzthiazoline-6-sulfonic acid). This assay, which employs an enantioselective alcohol oxidase as a reporter enzyme, was used to screen a panel of 17 KREDs in only 10 min using less than 0.5 mg substrate.
No takes yet. Share an insight, caveat, or question.
Truppo et al. (2008) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: