Reagentless enzymatic sensors based on carbon-paste electrodes containing ruthenium mediators for the on-line determination of glycerol

A new design is proposed for biosensors based on carbon-paste electrodes enriched with ruthenium-containing acyclic compounds. Two biologically sensitive elements, a fragment of the cellular wall of Gluconobacter sp.33 containing a new enzyme PQQ-dependent glycerol dehydrogenase and a commercially available preparation of NAD-dependent glycerol dehydrogenase, are analyzed and compared. The activity of biologically sensitive preparations is studied using artificial electron carriers represented by ruthenium-containing acyclic and coordination compounds.