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Growth of Pseudomonas fluorescens, Flavobacterium deciduosum, and B. vulgatus (all of marine origin) in buffered nutrient broth at 37°, 20°, 5°, 0° and −3 °C. has been followed quantitatively by means of plate counts. Maximum crops were obtained at 5 °C. in all cases, and higher total crops at 0° and −3° than at 37° and 20°. Maximum crop yield is considered a better criterion for optimum growth temperature than the growth rate during logarithmic growth. These marine bacteria are therefore considered to be truly psychrophilic.
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E Hess (1933) studied this question.
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