Isolating vector-insert junctions from yeast artificial chromosomes.

T h e physical mapping of complex genomes can be achieved by assembling contigs of yeast artificial chromosomes (YACs).In the early stages of map construction, randomly generated markers can be used to screen YAC libraries, order the clones, and assemble the contigs.However, as the maps increase in size, this process becomes less efficient, as randomly generated markers are less likely to add new clones to the ends of 3:141-150 9by Cold Spring Harbor Laboratory