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May 30, 2014Emerging infectious diseases253 citationsOpen Access

Genetic Variation among African Swine Fever Genotype II Viruses, Eastern and Central Europe

CGCarmina GallardoJFJovita Fernández-PiñeroVPV. Pelayo

Key Result

ASFV isolates from Lithuania and Poland shared 100% nucleotide identity with eastern European isolates in standard markers but contained a unique tandem repeat sequence insertion identical to isolates from Belarus and Ukraine.

Study Design

Type

Observational (n=25)

Structured PICO

P
Population
25 African swine fever virus isolates collected from wild boars and domestic pigs in Eastern Europe and the Caucasus region between 2007 and 2014.
E
Exposure
Genetic sequencing of p72, p54, CVR, and the intergenic region between I73R and I329L
O
Outcome
Genetic variation and identification of tandem repeat sequence (TRS) insertions

Genetic analysis of a novel tandem repeat sequence marker confirmed that the 2014 African swine fever virus outbreaks in Poland and Lithuania likely originated from Belarus.

Limitations

  • Requires additional sequence analysis of ASFVs currently circulating in Russian regions bordering Belarus and Ukraine to fully understand the evolution and spread of ASFV in this region.
  • Requires additional sequence analysis of ASFVs currently circulating in Russian regions bordering Belarus and Ukraine to fully understand evolution and spread

Abstract

African swine fever virus (ASFV) was first reported in eastern Europe/Eurasia in 2007. Continued spread of ASFV has placed central European countries at risk, and in 2014, ASFV was detected in Lithuania and Poland. Sequencing showed the isolates are identical to a 2013 ASFV from Belarus but differ from ASFV isolated in Georgia in 2007.

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Cite This Study

Gallardo et al. (2014) conducted an observational in African Swine Fever (n=25). Genetic sequencing of ASFV isolates was evaluated on Nucleotide sequence identity and tandem repeat sequence (TRS) variations. ASFV isolates from Lithuania and Poland shared 100% nucleotide identity with eastern European isolates in standard markers but contained a unique tandem repeat sequence insertion identical to isolates from Belarus and Ukraine.

synapsesocial.com/papers/6aa01d53516c2ec66e7c7810https://doi.org/10.3201/eid2009.140554
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