Site‐Specific Incorporation of Unnatural Amino Acids into Proteins

Unnaturally useful: The ability to incorporate unnatural amino acids into proteins (see scheme) has a significant impact on structure–function studies of proteins. Recently, the technique has been applied to proteins in vivo and in eukaryotic cells, thereby providing great prospects for protein engineering. A basic introduction to the field, together with recent developments and case-studies, is covered in this Minireview.