Key result
Simulated storage conditions had none to non-significant effects on the serological status of pig sera used for ASFV detection, whereas hemolyzed sera showed changes proportional to hemolysis degree.
Why the study?
Regulatory guidance and published data were lacking regarding storage conditions, shelf life, and their effects on the serological status of pig sera tested for anti-ASFV antibodies by ELISA.
Population
ASFV seropositive and seronegative pig sera
Comparison
Different storage temperatures and shelf life conditions
Design
Experimental laboratory study
Authors
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Storage minimally affects non-hemolyzed pig sera for ASFV ELISA; leaves open validation of hemolysis thresholds in field diagnostics.
Storage conditions have minimal effect on ASFV serodiagnosis by ELISA in non-hemolyzed pig sera, but hemolyzed sera show significant changes depending on hemolysis degree and storage duration.
Shotin et al. (2021) studied African swine fever (ASFV). Storage conditions (temperature and shelf life) was evaluated on Serological status of pig sera when tested by INgezim PPA Compac ELISA. Simulated storage conditions had none to non-significant effects on the serological status of pig sera used for ASFV detection, whereas hemolyzed sera showed changes proportional to hemolysis degree.
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