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Methods based on ion exclusion and gel permeation chromatography and enzymic degradation are reported for the assessment of the character, purity and amylopectin contents of amylose samples and their suitability for conversion by dyeing with Cibacron® Blue 3 G‐A to give coloured substrates for the assay of α‐amylase. The methods were also applicable to the assessment of the dyed materials. A new method for the estimation of α‐(1→6) linkages in starch samples, based on exhaustive hydrolysis with β‐amylase is reported. The susceptibilities of the free dye and dyed soluble amylose to acid and alkali are described along with their application to the measurement of the dye loading of dyed amyloses.
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John F. Kennedy (1976) studied this question.
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