Adsorption Reaction Constants between Nanosilica/Nanodiamond and Lysozyme Molecule at pH = 11.0

Adsorption reaction constants of chicken egg lysozyme of 0–1000 nM prepared with potassium phosphate buffer solution (PPBS) of 7.0 mM and at pH = 11.0 on the surfaces of nanosilica and nanodiamond of diameter 100 nm by fluorescence method have been determined as 4.0 × 107 and 1.5 × 108 nM−1, respectively. The Langmuir isotherm can be well followed under the assumption of monolayer adsorption on both surfaces. The available surface areas per individual lysozyme molecule adsorbed on nanosilica and nanodiamond within the linear regions of Langmuir isotherms can be approximated with 2 and 10 nm2, respectively.