Electrochemical Experimental Investigation on Behavior and Mode of the Interaction Between DNA and (S)-2-(5-Fluorouracil-1-Acetyl) Amido -1,4 -Succinic Acid

The cyclic voltammetry and spectroscopy have been proved to be powerful electrochemical means for the elucidation of interaction betweens Deoxyribonucleic acid (DNA) and chemical anti-carcinogens. With the aim to elucidate the interaction between DNA and anti-carcinogenic 5-fluorouracil derivatives using the instruments and techniques above, the electrochemical behavior of (S)-2-(5Fluorouracil-1-Acetyl) amido -1,4 -succinic acid (5FU-ASP) and the electrostatic interaction of 5FUASP with DNA using Fe(CN)63-/4-as electroactive indicator of dominance were investigated in this 3−1 study. The binding equilibrium constant of interaction is estimated to be 1.1×10×Mand DNA modified gold electrodes were prepared by the dry adsorptive method. Based on the electrochemical and ultraviolet–visible spectrum data, the interaction mode of DNA with 5FU-ASP was further explored, and the results serve as good reference for studies on 5FU-ASP targeting DNA strands or base pairs in the natural environment of living cells in vivo.