Phosphorylation‐Dependent Kinase–Substrate Cross‐Linking

Pinning down kinase substrates: The identification of substrates of a particular kinase is fundamental to the elucidation of cell-signaling cascades. This problem has now been addressed by the title approach involving kinase-catalyzed labeling coupled with photo-cross-linking (see scheme). When coupled with mass spectrometry analysis, this strategy can be used to determine the sites of phosphorylation as well as the effector kinase. ADP=adenosine 5′-diphosphate.