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July 30, 2016International Journal of Molecular SciencesOpen Access

A Next-Generation Sequencing Approach to Identify Gene Mutations in Early- and Late-Onset Hypertrophic Cardiomyopathy Patients of an Italian Cohort

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Why the study?

Does Next-Generation Sequencing yield different mutation detection rates in early-onset versus late-onset hypertrophic cardiomyopathy patients?

Population

70 patients with hypertrophic cardiomyopathy from an Italian cohort, including 35 with early-onset and 35…

Design

Cross-sectional

Key result

Next-Generation Sequencing yielded a significantly higher mutation detection rate in early-onset compared to late-onset hypertrophic cardiomyopathy patients (85.7% vs 22.9%, p<0.0001).

Authors

SRSperanza RubattuCBCristina BozzaoEPErmelinda Pennacchini

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Overview

NGS panels may boost diagnostic yield in early-onset HCM with family history; supports refined selection criteria but leaves broader adoption open pending validation.

Study Design

Type

Cohort (n=70)

Structured PICO

Does Next-Generation Sequencing yield different mutation detection rates in early-onset versus late-onset hypertrophic cardiomyopathy patients?

P
Population
70 hypertrophic cardiomyopathy patients from an Italian cohort, comprising 35 with early (≤25 years) and 35 with late (≥65 years) disease onset.
E
Exposure
Next-Generation Sequencing (NGS) panel of 17 HCM-related genes using PGM IonTorrent
O
Outcome
Mutation detection ratesurrogate

Main Result

Absolute Event Rate: 85.7% vs 22.9%

p-value: p=< 0.0001

Next-Generation Sequencing provides a high diagnostic yield in early-onset HCM and those with a family history, suggesting targeted genetic testing is highly effective in these subgroups.

Cite This Study

Rubattu et al. (2016) conducted a cohort in Hypertrophic cardiomyopathy (HCM) (n=70). Early disease onset (≤25 years) vs. Late disease onset (≥65 years) was evaluated on Mutation detection rate (p=< 0.0001). Next-Generation Sequencing yielded a significantly higher mutation detection rate in early-onset compared to late-onset hypertrophic cardiomyopathy patients (85.7% vs 22.9%, p<0.0001).

synapsesocial.com/papers/6aa02f87ec8c11126edcdfafhttps://doi.org/10.3390/ijms17081239
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