In exploring an effective and reliable karyotyping method in Brassica crop plants,Brassica oleraceawas successfully karyotyped using genomicin situhybridization (GISH).B. oleraceagenomic DNA was labelled as probe using DIG-high prime mix kit, withB. rapagenomic DNA acting as blocking agent. Specific fluorescent signals were detected on each pair of homologous chromosomes, and nine pairs of chromosomes ofB. oleraceawere clearly identified according to the signal characteristics. A practical and accurate method for conducting karyotyping of small chromosomes has been demonstrated.
No takes yet. Share an insight, caveat, or question.
Xiao-dan et al. (2009) studied this question.
Synapse has enriched 5 related papers. Consider them for comparative context: