A. Ait Adoubela, S. Guenub*, C. Elfakira & M. Dreuxa a Université d'Orléans , CNRS UPRES-A 6005, BP 6759, Orléans, Cedex 2, 45067, France b Antenne Scientifique Universitaire de Chartres (Université d'Orléans) , 21 rue de Loigny la Bataille, Chartres, 28000, France * Separations of small peptides (di-, tri- and tetrapeptides) using ion-pair chromatography and evaporative light scattering detection have been investigated with a porous graphitic carbon stationary phase, Hypercarb®. Five perfluorinated carboxylic acids (trifluoroacetic (TFA), pentafluoropropanoic (PFPA), heptafluorobutanoic (HFBA), nonafluoropentanoic (NFPA) and tridecafluoroheptanoic (TDFHA) acids) have been evaluated as ion-pairing agents. Influence of the percentage of organic modifier, concentration, and chain length of the ion-pairing agent on the retention factors, k, has been examined. Equilibration times were also studied. Separations of dipeptides or tri- and tetrapeptides are presented using HFBA and NFPA at 20 mM and TDFHA at 2.5 mM.
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Adoubel et al. (2000) studied this question.
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