Key result
Column chromatography of Venezuelan equine encephalomyelitis viruses on calcium phosphate gel demonstrated distinct elution profiles among antigenic subgroups.
Column chromatography on calcium phosphate gel provides a reproducible method to examine biochemical characteristics and relatedness of VEE virus strains.
May differentiate VEE strains biochemically; leaves open utility for surveillance or pathogenesis studies.
Column chromatography of selected Venezuelan equine encephalomyelitis (VEE) viruses on calcium phosphate gel offered a simple and reproducible method for examination of biochemical characteristics and relatedness of strains within the VEE complex. Members of antigenic subgroup I demonstrated a series of elution profiles within a narrow range of 0.22 to 0.25 M phosphate buffer. Members of antigenic subgroups II, III, and IV differed substantially among themselves and viruses of antigenic subgroup I. These differences in elution behavior may contribute to understanding of observed differences in biological behavior and antigenic variation among VEE viruses.
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Pedersen et al. (1972) studied Venezuelan equine encephalomyelitis (VEE) viruses. Column chromatography on calcium phosphate gel was evaluated on Elution profiles. Column chromatography of Venezuelan equine encephalomyelitis viruses on calcium phosphate gel demonstrated distinct elution profiles among antigenic subgroups.
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