Key result
In isolated rat lungs, the magnitude of the decrease in pulmonary vascular resistance in response to acetylcholine during ongoing hypoxic pulmonary vasoconstriction was significantly smaller in the bleomycin-treated group compared to the saline-treated group (p <0.05).
Why the study?
Does intratracheal bleomycin impair the vasodilator response to acetylcholine during alveolar hypoxia in isolated rat lungs?
Does intratracheal bleomycin impair the vasodilator response to acetylcholine during alveolar hypoxia in isolated rat lungs?
p-value: p=<0.05
In a rat model of bleomycin-induced lung injury, the pulmonary vasodilator response to acetylcholine during hypoxia is impaired, suggesting endothelial dysfunction.
Impaired acetylcholine response indicates endothelial dysfunction in bleomycin lung injury; leaves open translation to human pulmonary vascular disease.
We tested the hypothesis that during alveolar hypoxia the vasodilator response to acetylcholine (ACh) is impaired in lungs injured by intratracheal bleomycin. Isolated rat lungs were ventilated with normoxic (21% O2) or hypoxic (2% O2) gas and perfused with homologous blood. The effect of ACh on pulmonary vascular resistance was estimated during ongoing hypoxic pulmonary vasoconstriction (HPVC) after the temporal pattern of HPVC was established. The magnitude of the decrease in pulmonary vascular resistance was significantly smaller in the bleomycin-treated group (group B, n = 7) than in the saline-treated group (group S, n = 7) (p less than 0.05). The magnitude of HPVC itself did not differ between groups. The pulmonary vascular reactivities to angiotensin II and KCl were similar in the two groups (group S, n = 4, group B, n = 5). At any given transpulmonary pressure the lung volumes of group B (n = 5) were significantly smaller than those of group S (n = 4) (p less than 0.01). We conclude that vascular dilation in response to ACh during ongoing HPVC was impaired in bleomycin-injured rat lungs.
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Sato et al. (1990) studied Interstitial pulmonary fibrosis (bleomycin-induced model) (n=44). Intratracheal bleomycin vs. Intratracheal saline was evaluated on Magnitude of decrease in pulmonary vascular resistance (ΔPpa) in response to acetylcholine during ongoing hypoxic pulmonary vasoconstriction (p=<0.05). In isolated rat lungs, the magnitude of the decrease in pulmonary vascular resistance in response to acetylcholine during ongoing hypoxic pulmonary vasoconstriction was significantly smaller in the bleomycin-treated group compared to the saline-treated group (p <0.05).
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