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A homogenous assay of FAD using a binding between glucose oxidase (apo‐GOD) and FAD labeled with an electroactive compound was developed. Because daunomycin is sensitively detected with voltammetry, daunomycin was connected to FAD with a cross‐linker. The peak current decreased due to the apo‐GOD‐labeled FAD binding. Competitive reaction to the apo‐GOD between FAD and the labeled FAD produces the increase of peak current. Accordingly, FAD is detected on the basis of the reaction. The merit of this method is that the influence from FMN and riboflavin in the measurement of FAD can be suppressed by the high selective binding.
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Sugawara et al. (2006) studied this question.
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