Isolation and Characterization of Glyphosate-Tolerant Bacteria from a Local Coffee Plantation in Espírito Santo, Brazil

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High Resolution Image Download MS PowerPoint Slide This study aimed to isolate glyphosate-tolerant bacteria and characterize their plant-beneficial traits. Using a bioprospecting and glyphosate enrichment methodology, we isolated 35 bacterial strains that were tested for tolerance to glyphosate on solid and liquid media (0, 11, 22, 44, and 88 g L –1 of glyphosate). Sixteen isolates with high tolerance to glyphosate (≥22 g L –1 ) were identified and characterized by their ability to fix atmospheric nitrogen, solubilize inorganic phosphate, and produce indole-3-acetic acid (IAA). After similarity analysis and compatibility testing, a consortium composed of three bacteria ( Priestia sp.; Achromobacter sp.; Pseudomonas sp.) were selected to be applied in a model plant (tomato) and Arabica coffee beans. The bacterial consortium promoted the germination (96.6%) and development of tomato seedlings, while also degrading residual glyphosate (31.25%) in coffee beans, as evidenced by the reduced levels of the herbicide. These results demonstrate the bacterial consortium’s potential to reduce glyphosate residues and sustainably increase plant productivity.