Key result
Proteomic profiling of the human RCMH cell line identified over 6200 proteins with enrichment of muscle function proteins compared to HeLa and U2OS cells, confirming its suitability as an in vitro model.
The human RCMH cell line demonstrates myoblastic properties and is a suitable in vitro model for studying muscle function and myopathic disorders.
Studying (neuro)muscular disorders is a major topic in biomedicine with a demand for suitable model systems. Continuous cell culture (in vitro) systems have several technical advantages over in vivo systems and became widely used tools for discovering physiological/pathophysiological mechanisms in muscle. In particular, myoblast cell lines are suitable model systems to study complex biochemical adaptations occurring in skeletal muscle and cellular responses to altered genetic/environmental conditions. Whereas most in vitro studies use extensively characterized murine C2C12 cells, a comprehensive description of an equivalent human cell line, not genetically manipulated for immortalization, is lacking. Therefore, we characterized human immortal myoblastic RCMH cells using scanning (SEM) and transmission electron microscopy (TEM) and proteomics. Among more than 6200 identified proteins we confirm the known expression of proteins important for muscle function. Comparing the RCMH proteome with two well-defined nonskeletal muscle cells lines (HeLa, U2OS) revealed a considerable enrichment of proteins important for muscle function. SEM/TEM confirmed the presence of agglomerates of cytoskeletal components/intermediate filaments and a prominent rough ER. In conclusion, our results indicate RMCH as a suitable in vitro model for investigating muscle function-related processes such as mechanical stress burden and mechanotransduction, EC coupling, cytoskeleton, muscle cell metabolism and development, and (ER-associated) myopathic disorders.
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Kollipara et al. (2016) studied Neuromuscular disorders. Human RCMH cell line vs. HeLa and U2OS cell lines was evaluated on Proteome and ultrastructural characteristics. Proteomic profiling of the human RCMH cell line identified over 6200 proteins with enrichment of muscle function proteins compared to HeLa and U2OS cells, confirming its suitability as an in vitro model.
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