Polythiophene gold nanoparticles composite film for application to glucose sensor

Regioregular poly‐3‐hexylthiophene (regP3HT) and dithiobissuccinimidyl propionate (DTSP) have been used to prepare bifunctionalized gold nanoparticles (Bf AuNPs). Processable properties of regP3HT have been used to obtain regP3HT‐AuNPs‐DTSP film on a gold‐coated glass surface and the succinimidyl group of DTSP in this regP3HT‐AuNPs‐DTSP/Au electrode has been utilized for covalent immobilization of glucose oxidase (GOx). The UV‐visible (UV‐vis), scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopic studies have been used to characterize regP3HT‐AuNPs‐DTSP/Au and GOx‐regP3HT‐AuNPs‐DTSP/Au electrode, respectively. This GOx‐regP3HT‐AuNPs‐DTSP/Au bioelectrode shows response time of 10 s, linearity from 25 to 300 mg/dL of glucose and the value of Michaelis‐Menten constant as 5.85 mM (105.3 mg/dL).