FtmPT2, an N ‐Prenyltransferase from Aspergillus fumigatus , Catalyses the Last Step in the Biosynthesis of Fumitremorgin B

Last, but not least: The ftmPT2 gene, which was identified in a biosynthetic gene cluster for fumitremorgins in Aspergillus fumigatus, was over-expressed in Saccharomyces cerevisiae. The purified soluble dimeric His6–FtmPT2 was characterised biochemically. Structure elucidation of the enzymatic product showed that FtmPT2 catalyses the prenylation of 12,13-dihydroxyfumitremorgin C in the presence of dimethylallyl diphosphate at position N1 of the indole ring and so functions as an N-prenyltransferase (see scheme).