An ovarian weight increase in the hypophysectomized rat treated JLX. with diethylstilbestrol was first reported by Williams (1940). Estradiol dipropionate has been shown by Pencharz (1940) to produce a similar, but less marked, effect. Simpson and collaborators (1941), and Gaarenstroom and deJongh (1946) have demonstrated that this gain in ovarian mass in due to an increased number of healthy, solid, medium-sized follicles. The present study is concerned with quantitative aspects of the above phenomenon. METHODS Female rats of the Holtzman strain 24–25 days of age, weighing 45–50 gm. were hypophysectomized using the parapharyngeal approach. A few animals were also bilaterally adrenalectomized at the time of hypophysectomy. Injections were begun promptly after hypophysectomy in order to minimize the ovarian atrophy which occurs soon after removal of the pituitary gland. Estrogen was administered daily for 4 days in equally divided doses and the animals were killed by cervical fracture 48 hours after the last injection, 120 hours after hypophysectomy.
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Payne et al. (1955) studied this question.