The photoprotein in the luminous squid, Symplectoteuthis oualaniensis, was extracted in high salt solutions at low temperatures. It was partially purified to show bioluminescence when warmed to room temperature. To the resulting protein solution after this luminescence, three solutions containing dehydrocoelenterazine, its DTT adduct and GSH adduct were added to observe immediate light, respectively. These recorded the first reconstruction of the luminous system in this squid. Coelenterazine, however, gave only weak light in 2 h after addition.
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Takahashi et al. (1994) studied this question.
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