Genetic Mapping of SSR Markers in Eight Soybean Chromosomes Based on F2 Population B3462 x B3293

Genetic Mapping of SSR Markers in Eight Soybean Chromosomes Based on F2 Population B3462 x B3293. I Made Tasma, Ahmad Warsun, Dani Satyawan, Saptowo J. Pardal, and Slamet. Aluminum toxicity is one of the main contrains for cultivating soybean in acid soils. Genetic Hak Cipta © 2011, BB-Biogen mapping of SSR markers is one step for detecting aluminumtoxicity tolerant QTLs in soybean. Another step is to phenotype the same population at various aluminum-toxicity environments. The objectives of this study were to analyze the segregation of SSR markers in progenies of an F2 population and map the markers in 8 soybean chromosomes. The F2 population was previously developed by crossing the Al-tolerant parent B3462 and the Al-sensitive parent B3293. Polymorphic SSR markers in the parents were used to PCR amplify DNA of the 100 F2 progenies. PCR products were separated using agarose or polyacrylamide gels. A Chi-Square test was done with a null hypothesis that progenies segregated in a 1 : 2 : 1 ratio. Results showed that 125 SSR markers were polymorphics in the parents. Out of 125 polymorphic markers, 122 were segregated in the progenies of the F2 population. Among the segregating markers, 114 were segregated in a 1 : 2 : 1 ratio. Only 8 markers (5.6%) did not follow the 1 : 2 : 1 ratio. One hundred and nineteen SSR markers were mapped in 8 soybean chromosomes. These include 18 markers in chromosome A2, 10 in B1, 16 (C1), 16 (F), 10 (G), 23 (J), 16 (L), and 10 (N). Total genetic maps covered was 1,194.8 cM with average map distances between two adjacent markers of 10.7 cM. Further SSR marker enrichment is required to fill in the gaps of several chromosomal regions. Genetic maps presented in this study should be useful for detection of Al-toxicity tolerant QTLs in soybean.