Key result
Cysteine mutagenesis reveals the IV/S4-S5 loop regulates fast inactivation in muscle Na+ channels.
Population
Human embryonic kidney cells expressing adult human muscle Na+ channel mutants
Comparison
Intracellular application of sulfhydryl reagents… vs Wild-type channels and absence of sulfhydryl…
Design
Preclinical
Authors
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No immediate clinical implications for channelopathies; extends structural insights into Na+ channel inactivation for future studies.
The IV/S4-S5 loop plays an important role in fast inactivation of the muscle Na+ channel, suggesting an alpha-helical structure with distinct stabilizing and destabilizing clusters.
Lerche et al. (1997) studied this question. Cysteine mutagenesis and sulfhydryl reagents (MTSET and MTSES) vs. Wild-type channels / absence of sulfhydryl reagents was evaluated on Fast inactivation gating of Na+ channels. Cysteine mutagenesis and sulfhydryl reagent application revealed that the IV/S4-S5 loop plays an important role in fast inactivation of the muscle Na+ channel, suggesting an alpha-helical structure.
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