What question did this study set out to answer?

Develop a catalyst-free, one-pot dual-functionalization platform by coupling the nonhydrolysis Staudinger reaction with Staudinger-Bertozzi or SPAAC ligations without protective groups.

January 1, 2019Organic & Biomolecular Chemistry

The one-pot nonhydrolysis Staudinger reaction and Staudinger or SPAAC ligation

View Full Paper

Ask AI

Bookmark

Authors

LCLonghuai ChengNankai University XKXueying KangNankai University DWDan WangCentral South University

Discussion

Member takes

Implication

Experimental study demonstrates one-pot dual bioorthogonal functionalization via tandem Staudinger and SPAAC reactions in living cells, suggesting versatile tools for biomolecular labeling.

Key Points

Develop a catalyst-free, one-pot dual-functionalization platform by coupling the nonhydrolysis Staudinger reaction with Staudinger-Bertozzi or SPAAC ligations without protective groups.
Engineered a bisazido linker possessing both a tetrafluorinated aromatic azide and an alkyl azide for selective sequential labeling.
Evaluated relative reaction rates and kinetics using HPLC analysis and fluorescence assays across reaction partners.
Synthesized molecular conjugates in one-pot configurations and performed dual-color bioimaging of a FRET-based dyad in living cells.
The nonhydrolysis Staudinger reaction proceeded significantly faster on the tetrafluorinated aromatic azide than Staudinger-Bertozzi or SPAAC ligations on the alkyl azide.
Tandem one-pot assembly delivered molecular beacon 7 via Staudinger-Bertozzi ligation in 32% recovery yield and compound 8 via SPAAC ligation in 59% recovery yield.
Tandem nonhydrolysis Staudinger and SPAAC reactions successfully generated an active FRET dyad directly inside living cells.

Cite This Study

Cheng et al. (2019) studied this question.

synapsesocial.com/papers/6aa0fcaeb6ff41cbc45b88ea https://doi.org/10.1039/c9ob00528e

View Full Paper

Ask AI

Bookmark

January 1, 2019Organic & Biomolecular Chemistry

The one-pot nonhydrolysis Staudinger reaction and Staudinger or SPAAC ligation

View Full Paper

Ask AI

Bookmark

Authors

LCLonghuai ChengNankai University XKXueying KangNankai University DWDan WangCentral South University

Discussion

Member takes

Implication

Experimental study demonstrates one-pot dual bioorthogonal functionalization via tandem Staudinger and SPAAC reactions in living cells, suggesting versatile tools for biomolecular labeling.

Key Points

Develop a catalyst-free, one-pot dual-functionalization platform by coupling the nonhydrolysis Staudinger reaction with Staudinger-Bertozzi or SPAAC ligations without protective groups.
Engineered a bisazido linker possessing both a tetrafluorinated aromatic azide and an alkyl azide for selective sequential labeling.
Evaluated relative reaction rates and kinetics using HPLC analysis and fluorescence assays across reaction partners.
Synthesized molecular conjugates in one-pot configurations and performed dual-color bioimaging of a FRET-based dyad in living cells.
The nonhydrolysis Staudinger reaction proceeded significantly faster on the tetrafluorinated aromatic azide than Staudinger-Bertozzi or SPAAC ligations on the alkyl azide.
Tandem one-pot assembly delivered molecular beacon 7 via Staudinger-Bertozzi ligation in 32% recovery yield and compound 8 via SPAAC ligation in 59% recovery yield.
Tandem nonhydrolysis Staudinger and SPAAC reactions successfully generated an active FRET dyad directly inside living cells.

Cite This Study

Cheng et al. (2019) studied this question.

synapsesocial.com/papers/6aa0fcaeb6ff41cbc45b88ea https://doi.org/10.1039/c9ob00528e

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark