Cerebral Anoxia: Protein Metabolism During Recovery in In Vitro Model

Amino acid incorporation into proteins of rabbit brain slices was studied during the recovery stage after various anoxic periods in an in vitro experimental model. There was a distinct difference between anoxic periods up to five minutes and those beyond ten minutes. In the former, inhibition of amino acid incorporation was of lesser degree and was reversible. On the other hand, more than 50% inhibition was observed following anoxic periods of 30 minutes and this inhibition was irreversible. Amino acid incorporation into proteins was more sensitive to anoxia than was amino acid transport into brain slices.