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November 25, 2022Journal of OncologyOpen Access

ANGPTL4 Regulates Lung Adenocarcinoma Pyroptosis and Apoptosis via NLRP3 8 Signaling Pathway to Promote Resistance to Gefitinib

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Why the study?

With EGFR-TKI resistance increasing, the role of ANGPTL4 in regulating gefitinib resistance in PC9/GR non-small-cell lung cancer needed investigation.

Does ANGPTL4 inhibition reduce gefitinib resistance in lung adenocarcinoma cells?

Population

A549, PC9, H1975, BEAS-2B, and PC9/GR cells and mouse transplantation tumors

Comparison

ANGPTL4 knockdown or overexpression vs controls

Design

In vitro and in vivo preclinical laboratory study

Key result

Interfering with ANGPTL4 expression in PC9/GR cells promoted sensitivity to gefitinib and mediated the NLRP3/ASC/Caspase 8 pathway to induce cell scorching and apoptosis.

Authors

YFYue FangXLXuan LiHCHao Cheng

Discussion

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Member takes

Overview

ANGPTL4 inhibition may overcome gefitinib resistance in preclinical lung adenocarcinoma models; leaves open clinical applicability.

Structured PICO

Does ANGPTL4 inhibition reduce gefitinib resistance in lung adenocarcinoma cells?

P
Population
Lung adenocarcinoma cell lines (A549, PC9, H1975, BEAS-2B, PC9/GR) and mouse transplantation tumor models
I
Intervention
Inhibition of ANGPTL4 expression using shRNA or overexpression of ANGPTL4
C
Comparator
Control cells/mice without ANGPTL4 interference
O
Outcome
Gefitinib resistance, cell proliferation, migration, pyroptosis, and apoptosissurrogate

ANGPTL4 promotes gefitinib resistance in lung adenocarcinoma cells by inhibiting pyroptosis via the NLRP3/ASC/Caspase 8 pathway, suggesting it as a potential therapeutic target.

Cite This Study

Fang et al. (2022) studied Gefitinib resistance in non-small-cell lung cancer (NSCLC). ANGPTL4 interference/knockdown vs. Control was evaluated on Gefitinib sensitivity, cell proliferation, migration, scorching, and apoptosis. Interfering with ANGPTL4 expression in PC9/GR cells promoted sensitivity to gefitinib and mediated the NLRP3/ASC/Caspase 8 pathway to induce cell scorching and apoptosis.

synapsesocial.com/papers/6aa10a658eabfe21f24f8504https://doi.org/10.1155/2022/3623570
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