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The influence of frozen storage on the ultrastructural integrity of Atlantic cod muscle tissue membranes was investigated following three methods of freezing and retention for 12 wk at ‐ 12°C and ‐ 35°C. In comparison to controls (0 wk), extensive membrane condensation associated with sarcoplasmic reticulum was apparent in samples stored for 12 wk at ‐ 12°C. The effects were observed to a much lesser extent in samples retained for 12 wk at – 35°C. It was apparent that cryogenic, plate or blast freezing techniques showed no measurable influence on the observed membrane condensation and that such ultrastructural changes resulted as a consequence of relatively high (–12°C) subfreezing temperatures.
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ABLETT et al. (1992) studied this question.
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